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NMN and NAD+: A 60-Day Dose-Ranging Randomized Trial

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In an 80-person, 60-day randomized trial, NMN at 300, 600 or 900 mg/day increased blood NAD and six-minute walking...

In an 80-person, 60-day randomized trial, NMN at 300, 600 or 900 mg/day increased blood NAD and six-minute walking distance compared with placebo. HOMA-IR did not differ significantly. A blood-based age estimate remained stable in NMN groups while increasing in placebo; this is not evidence of longer life or reversal of aging.

Key Findings

  • The trial randomized 80 healthy middle-aged adults to placebo or 300, 600 or 900 mg NMN daily for 60 days; all 80 completed the trial.
  • Blood NAD concentrations increased versus placebo at days 30 and 60 in allNMN groups, with the highest concentrations in the 600 mg and 900 mg groups.
  • Six-minute walking distance improved versus placebo. This performance measure should not be relabeled as a measured increase in aerobic capacity.
  • HOMA-IR showed no statistically significant difference versus placebo at day 60.
  • The Aging.Ai blood-age estimate stayed unchanged in NMN groups and increased in placebo. The study did not measure lifespan or establish age reversal.
  • No safety issues emerged during this 60-day trial. Short follow-up does not establish long-term safety.

Study Details

The efficacy and safety of β-nicotinamide mononucleotide (NMN) supplementation in healthy middle-aged adults: a randomized, multicenter, double-blind, placebo-controlled, parallel-group, dose-dependent clinical trial
Yi L, Maier AB, Tao R, Lin Z, Vaidya A, Pendse S, et al. — Geroscience (2023)
Placebo-controlled 60-day study of 300, 600 and 900 mg/day: blood NAD and walking distance increased, HOMA-IR did not differ, and the exploratory blood-age estimate remained stable in treated groups.
80 participantsModerate

Practical Takeaway

NMN increased a blood NAD biomarker in this short trial, with a signal in walking performance. The studied doses and population should be kept distinct from a separate 250 mg/day trial in older men. Neither study establishes a longevity benefit or a routine dose for healthy aging.

Summary

A placebo-controlled trial of 80 healthy middle-aged adults examined 300, 600 and 900 mg/day of NMN for 60 days, measuring blood NAD, walking performance and exploratory aging biomarkers.

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Frequently Asked Questions

Does NMN increase blood NAD in humans?

Yes. This trial found higher blood NAD at days 30 and 60 with 300, 600 and 900 mg/day than with placebo. Blood NAD is a biomarker; its increase does not itself establish a clinical longevity benefit.

Did this trial show that NMN reverses aging?

No. A blood-based age calculator remained stable in the NMN groups while increasing in placebo. The trial did not assess lifespan, disease prevention or reversal of biological aging.

Is the 250 mg trial the same study?

No. Igarashi and colleagues studied 250 mg/day in healthy older men for 6 or 12 weeks and reported higher NAD plus nominal improvements in gait speed and left-grip performance. Yi and colleagues studied 80 middle-aged adults at 300-900 mg/day for 60 days.

Does the research establish long-term safety?

The 60-day trial found no safety signal in its monitored outcomes. Longer-term and broader-population safety cannot be concluded from this short study.

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Supplement Guide

NMN

NMN raises blood biomarkers, but anti-aging benefit remains unproven. Short trials do not establish long-term safety or a safe upper dose. [2,10,11,14,15]

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References

  1. Igarashi M, Nakagawa-Nagahama Y, Miura M, Kashiwabara K, Yaku K, Sawada M, et al. (2022). Chronic nicotinamide mononucleotide supplementation elevates blood nicotinamide adenine dinucleotide levels and alters muscle function in healthy older men. npj Aging. DOI PubMed
  2. Yi L, Maier AB, Tao R, Lin Z, Vaidya A, Pendse S, et al. (2023). The efficacy and safety of β-nicotinamide mononucleotide (NMN) supplementation in healthy middle-aged adults: a randomized, multicenter, double-blind, placebo-controlled, parallel-group, dose-dependent clinical trial. GeroScience. DOI PubMed